Syllabus for Roster(s):
- 13F CHEM 4411-001 (CGAS)
- 13F CHEM 4411-002 (CGAS)
- 13F CHEM 4411-003 (CGAS)
CHEM4411 Course Info
CHEM 4411 / 4421 — Biological Chemistry Lab I and II
Course Instructors
Prof. Cameron Mura (PLSB 116)
Prof. Linda Columbus (PLSB 106)
Dr. Carol Price (PLSB 120)
Intern Instructor: Ryan Lo
Fall 2013 – Meeting Times and Places
Lecture Friday 2 – 2:50 PM CHM Rm. 304
Lab Tue, Wed, or Thurs 2 – 6:00 PM CHM Rm. 315 or 412
Office Hours
TAs: Monday 3:00 PM Lab Rm. 412
Friday 11:00 AM Lab Rm. 412 (subject to change)
Professor Friday 3:00 PM PLSB 106/116
Spring 2014 – Meeting Times and Places
Lecture Monday 12 – 12:50 CHM 304 (Tentative)
Teaching assistants (TAs)
|
Catrina Campbell |
cac4ff@virginia.edu |
PLSB Rm 134 |
Thursdays, RM 315 |
|
Jason Kuhn |
jpk9cn@virginia.edu |
PLSB Rm 132 |
Tuesdays, RM 412 |
|
Kim Stanek |
kas4wk@virginia.edu |
PLSB Rm 132 |
Tuesdays, RM 315 |
|
Marissa Keiber |
mkk9bs@virginia.edu |
PLSB Rm 136 |
Wednesdays, RM 412 |
|
Sarah Bentley |
sfb4ug@virginia.edu |
TBA |
|
|
Ellen Speers |
ehs5t@virginia.edu |
TBA |
|
Required materials (both semesters):
· Text: “At the Bench: A Laboratory Navigator” by Kathy Barker
· This lab manual
· Lab Notebook with numbered pages, preferably grid printed (you will use the same notebook for both semesters)
· Materials provided on Collab site
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Proteins of Interest (POIs) |
|
|
|||
|
Accession # |
PDB ID |
Putative function |
Organism |
TA* |
Advisor*§ |
|
YP_194538.1 |
3f6t |
AspAT |
Lactobacillus acidophilus |
Marissa Kieber |
CP |
|
ZP_03625122.1 |
3p6k |
AspAT |
Streptococcus suis |
Marissa Kieber |
CP |
|
NP_207418.1 |
3ezs |
AT |
Helicobacter pylori |
Ellen Speers |
LC |
|
YP_263484.1 |
3ke3 |
Ser-Pyr AT |
Psychrobacter arcticus |
Kim Stanek |
CM |
|
NP_283882.1 |
3jtx |
AT |
Neisseria meningitidis |
Kim Stanek |
CM |
|
YP_002938516.1 |
3f0h |
AT |
Eubacteriium rectale |
Jason Kuhn |
CP |
|
NP_599493.1 |
3ppl |
AspAT |
Coyrnebacterium glutamicum |
Jason Kuhn |
CP |
|
NP_940074.1 |
3fdb |
Cystathionine β-lyase |
Corynebacterium diphtheriae |
Catrina Campbell |
CM |
|
YP_604413.1 |
3ez1 |
AT |
Deinococcus geothermalis |
Sarah Bentley |
LC |
|
YP_677612.1 |
3ffr |
Phosphoserine AT |
Cryptophaga hutchinsonii |
Catrina Campbell |
CM |
|
RER070207001803 |
3ele |
AT |
Eubacterium rectale |
Ellen Speers |
LC |
|
AAH25799.1 |
3kgw |
AT |
Mus musculus |
Sarah Bentley |
LC |
|
Grade distribution |
|
|
Overall laboratory performance |
100 |
|
Laboratory exercises and handouts |
|
|
Lab 1A and 1B assignment |
35 |
|
Lab 2 assignment |
50 |
|
Lab 3A and 3B assignment |
40 |
|
Lab 6 assignment |
50 |
|
Lab Reports |
|
|
Kinetics report |
100 |
|
Protein expression and purification report |
150 |
|
Protein concentration |
50 |
|
POI report/Lab 11 assignment questions and figures |
75 |
|
Pre-lab quizzes (best 10) |
100 |
|
Lab notebook/pre-lab assignments |
100 |
|
Final exam |
150 |
|
TOTAL |
1000 |
|
Final Letter Grades |
|
|
990 – 1000 |
A+ |
|
940 – 989 |
A |
|
900 – 939 |
A- |
|
880 – 899 |
B+ |
|
840 – 879 |
B |
|
800 – 839 |
B- |
|
780 – 799 |
C+ |
|
740 – 779 |
C |
|
700 – 739 |
C- |
|
600 – 699 |
D |
|
< 600 |
F |
At the end of the term, the averages of each lab section will be compared to the final exam averages and normalized for differences in grading between lab sections (different TAs).
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